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| Basmati Testing |
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| Basmati Verifiler™ Kit |
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| Description |
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Part No. |
| Basmati Verifiler™ Kit* |
100 tests |
BV81001 |
| Genescan 500 LIZ** |
800 reactions |
4322682 |
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| * Order from Labindia |
| ** Order from AB |
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World's first kit-based product for testing the authenticity of Basmati rice samples |
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Specific microsatellite profiles of loci common to all major Basmati varieties to distinguish traditional Basmati from evolved and non-Basmati unambiguously |
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"Single-tube assay" to co-amplify eight microsatellite loci |
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High throughput analysis enabled via five-dye fluorescent DNA technology |
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Integrated system including reagents, Applied Biosystems Genetic Analysis instrumentation, software and technical support |
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| Basmati (from the Hindi word for fragrant) is a special rice cultivated exclusively in the Indo-Gangetic plains. Basmati's reputation as premium long grain rice is known worldwide and is preserved in traditional varieties. Its high value stems from the grain's characteristic aroma in both the raw and cooked state. The grain also possesses a distinctive shape, which on cooking elongates to almost double its length whilst its width remains the same. Two thirds of the total Basmati exported to Europe and the Middle East comes from India. |
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| Consumer preference generates higher returns for traditional Basmati varieties leading to strong brand equity. Thus, the deliberate addition of non-Basmati rice to Basmati rice would offer a significant cost advantage to dishonest suppliers. Hence to protect the interests of consumers and rice-trading community in general, identification of genuine Basmati rice samples is vital. |
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| World's First Single-tube, Multiplex, Microsatellite Assay-based Kit for Basmati Authentication |
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| The Basmati Verifiler™ Kit is the world's first product for establishing the authenticity of Basmati rice samples via a molecular assay. The kit is manufactured and marketed by Labindia and was developed by a group of scientists from the Centre for DNA Fingerprinting and Diagnostics (CDFD) in Hyderabad, India. The kit uses a PCR amplification technique based on Simple Sequence Repeats (SSR) that provides the single most discriminating assay for Basmati genotyping. |
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| Speed and Throughput |
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| Analyzing different genetic markers usually calls for the amplification of products using separate PCRs with individual analysis for each marker. To simplify this process, the Basmati Verifiler Kit employs a robust, eight-locus multiplex PCR, which significantly reduces the preparation time and effort required for amplification and analysis. With the Basmati Verifiler Kit, one can perform a single multiplex PCR followed by capillary electrophoresis to test the authenticity of the rice sample. |
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| The Basmati Verifiler Kit uses unique and laboratory tested primer sequences. Allele sizes range between 70 to 350 base pairs for robust PCR amplification of all loci. When used with an AB platform, the kit provides an integrated approach, and the flexibility to choose from a wide range of Applied Biosystems (AB) Genetic Analyzers depending on the throughput requirement |
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| Kit Data Satisfies Recommendations of Indian and European Regulatory Agencies |
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| The data generated using the Basmati Verifiler Kit from the eight microsatellite loci also satisfy the recommendations of the government agencies regulating the Indian (APEDA) and European markets (Food Standard Agency, UK). Due to strict legislations governing the export market, the combination of loci included in the Basmati Verifiler Kit makes it the best technology available to establish Basmati authenticity before being labeled as such. |
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| Kit Workflow |
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| Kit Components |
| Each Basmati Verifiler Kit contains a pre-formulated PCR reaction mix, blended primer set along with pure Basmati DNA as a control. The kit provides sufficient reagents for 100 reactions. The PCR reaction components, primer sequences and amplification protocols have all been developed to provide specific, robust amplification. The kits are subjected to rigorous quality control testing to ensure reliable performance. |
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